Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.070459 |
Chromosome: | plastome |
Location: | 28832 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
CreCp.g802278 | 2717059,rpl5,ChreCp016 | 50S ribosomal protein L5; (1 of 2) PF00673 - ribosomal L5P family C-terminus (Ribosomal_L5_C) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTATACAATACAATATAGCAATTTATTTAGCGAGGATACAGTAAATA |
Internal bar code: | GCATGCGTTACCGAAGACACCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 770 |
LEAP-Seq percent confirming: | 33.871 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 41 |
LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCCTTCGGGCAAGTAAACT |
Suggested primer 2: | GCCACTGACGTCCACTACAA |