| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070460 |
| Chromosome: | chromosome 9 |
| Location: | 5602397 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409750 | CAX2 | (1 of 3) K07300 - Ca2+:H+ antiporter (chaA, CAX); CAX family cation antiporter, membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCTGTGGTAGCCGAGTCCGGCTCGGCTGCTGCTTTGTGCGAGCAGAT |
| Internal bar code: | AAGTTGGTCTTTCGCATCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1242 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACTGCAGCAGGAGAGT |
| Suggested primer 2: | TACGTGCTCATCTCCATCGC |