| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070461 |
| Chromosome: | chromosome 7 |
| Location: | 487219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315600 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | 5'UTR | |
| Cre07.g315650 | SMM23 | (1 of 1) 2.1.1.164 - Demethylrebeccamycin-D-glucose O-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTCCGGAGAGGGTGCGTGTCTGTGGATCCGAGCCAAGCCGAGACTGA |
| Internal bar code: | TAATCGGTCCGTGGCATGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1204 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCCGAGCATTTGTGACT |
| Suggested primer 2: | CGAGCAGCAGACACCCTAAA |