| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070468 |
| Chromosome: | chromosome 3 |
| Location: | 3761410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169400 | GAD1,UXS1 | (1 of 1) 4.1.1.35 - UDP-glucuronate decarboxylase / UDP-glucuronic acid decarboxylase; UDP-glucuronic acid decarboxylase for UDP-Xylose synthesis | 3'UTR |
| Cre03.g169450 | (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACACAAAGGGTCACATGATGAGCTCACGGAGTGACTTGGACAGGAA |
| Internal bar code: | TGCGAAGTCCCGTCCAAAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1611 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTGATCGGACTTGCGAC |
| Suggested primer 2: | TCACCAAGGTCAAGACGACG |