| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070479 |
| Chromosome: | chromosome 2 |
| Location: | 3865094 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101350 | RPL10A,RPL10a | Cytosolic 80S ribosomal protein L10a; (1 of 1) K02865 - large subunit ribosomal protein L10Ae (RP-L10Ae, RPL10A) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATATTATGCATGATGTCTTCCGCAGAAGAGGCACCGTGATGCCCACC |
| Internal bar code: | TATGTCGGAGAAGAGGAGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6288 |
| LEAP-Seq percent confirming: | 98.7342 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACCAGGGCTGCAATAA |
| Suggested primer 2: | CGCATTAGAGAGGGACCGAC |