| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070482 |
| Chromosome: | chromosome 9 |
| Location: | 2699412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801023 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACATACGCTGTACGGTTCCCTGGCGCCCTACCGTCCGCTGCTGCGGG |
| Internal bar code: | AGTGTACAGCTTCTGGTTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1666 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATCCCGCGTCTTAGATC |
| Suggested primer 2: | GAACTCCTCACACAGCTGCT |