Insertion junction: CLIP2.070485_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.070485
Chromosome:chromosome 12
Location:4692077
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g522550 FLA8,KIN2-1 Kinesin II Motor Protein; (1 of 2) K10394 - kinesin family member 3/17 (KIF3_17) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGCGAACACACGCCGCGCCCAGATGCAACCCTATCCCGCTCTTGAGA
Internal bar code:CTTCTGCCAGGCAGTAATATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2868
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTTTGGTCGTTGTGTGTC
Suggested primer 2:TTCTGGTTCGTGTCGGTCAG