| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.070500 |
| Chromosome: | chromosome 9 |
| Location: | 2383716 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393900 | GCH2 | Putative GTP cyclohydrolase; (1 of 2) 3.5.4.25 - GTP cyclohydrolase II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCATGCCTAGGGTAAATCACGAGTCCCGCGCCGCCGCTGCGCCACCG |
| Internal bar code: | TTGTAATGATCCTGACGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCACCCATGCAACAAAC |
| Suggested primer 2: | TAGCCTGTACGAGACCAGCT |