| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070505 |
| Chromosome: | chromosome 17 |
| Location: | 2294896 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713550 | HTR31,HTR22 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACTCGTGCCAGCCTGTCGCGCACGATGGAAGTGGGGCTCTGTGGGA |
| Internal bar code: | AGTCGCGGTCTAATTAATGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4223 |
| LEAP-Seq percent confirming: | 88.9764 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 127 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATAGTGGGATCGCAGGG |
| Suggested primer 2: | GACCATCATGCCCAAGGACA |