| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070508 |
| Chromosome: | chromosome 5 |
| Location: | 2257379 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238000 | (1 of 3) IPR006502//IPR011705 - Protein of unknown function PDDEXK-like // BTB/Kelch-associated | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATTTGATGCTCGGCTAGGGATTCGTTTTGTACATTCACGCTCCGCAT |
| Internal bar code: | AAGTGCTGCGTGAGCGTAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1215 |
| LEAP-Seq percent confirming: | 71.7949 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCATGCGCTAACGTAC |
| Suggested primer 2: | GGCTGTGCGTAGATTGCATG |