| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070518 |
| Chromosome: | chromosome 9 |
| Location: | 5621042 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409951 | RuvX homolog; (1 of 1) K07447 - putative holliday junction resolvase (ruvX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAATGCCCTGTATGCGATTCTGGTTGTCGGTTTCAACTCTGTACTCT |
| Internal bar code: | TCGCCAGGTGGGTGAGAGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1033 |
| LEAP-Seq percent confirming: | 23.6842 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATCCCTACCCATGCCCCA |
| Suggested primer 2: | AGCAGACTCTGGGGGACTAG |