| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070526 |
| Chromosome: | chromosome 7 |
| Location: | 5589887 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800856 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTACTTCGTTTTTCCCAGCTGATTTGTTTGGCGGCGGTGCCTGGTCG |
| Internal bar code: | ATTCCCGCACGGTTTAGTAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3248 |
| LEAP-Seq percent confirming: | 11.5942 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTACAGCCTAGCCCACA |
| Suggested primer 2: | ATCGCTACTGCTACTCGCAC |