| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070527 |
| Chromosome: | chromosome 12 |
| Location: | 1938614 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511640 | PIGX,PIGX1 | (1 of 1) PF08320 - PIG-X / PBN1 (PIG-X); co-factor of alpha-(1-4)-Mannosyltransferase, PIG-X | 3'UTR |
| Cre12.g511650 | (1 of 77) IPR001623 - DnaJ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTTACACACGTTGAGGGCAATCCGCCCAGACACCAGTCACCCGTGC |
| Internal bar code: | AGGGATGCCGGGTCGAGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2826 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATGGTGTTTGACGTGC |
| Suggested primer 2: | CAATGTTGGGTTGGGCTGTG |