| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070529 |
| Chromosome: | chromosome 12 |
| Location: | 1695611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486250 | ARL6,ARFA1A,FAP333 | (1 of 2) PTHR11711:SF181 - ADP-RIBOSYLATION FACTOR A1F; Flagellar Associated Protein 333 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATATCAGCATGCCTGCATGTGACTGGCTTCCCTTACTTCGCATTGT |
| Internal bar code: | AGGTCCGTGGGCGTCCTCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2757 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTCCAGGTTCCGACGTC |
| Suggested primer 2: | CAACTGCAAGCAGGTTGACC |