Insertion junction: CLIP2.070531_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.070531
Chromosome:chromosome 13
Location:1196480
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569850 AMT4,AMT1D Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) 3'UTR
Cre13.g569900 (1 of 100) IPR001810 - F-box domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATTTCACAACATGTAGGCTTCGTCACTTGCATTCACGTCAGGGCATCC
Internal bar code:ATTTCTAACTGGGAAGCTGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2072
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGGCTCAGTAACTGCAG
Suggested primer 2:AACTTGTTTCACTCACGCGC