| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070546 |
| Chromosome: | chromosome 8 |
| Location: | 710145 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360550 | ERM3,NIRK,NIRK1 | Copper-containing nitrite reductase, mitochondrial; (1 of 1) 1.7.2.1 - Nitrite reductase (NO-forming) / Pseudomonas cytochrome oxidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGGGGTCCTTTGTCGGACTGGGCCTGCTGCCGTAATGACTAAGCAAT |
| Internal bar code: | GAAATGCTAAAGGACCCATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTACAAGAACCCCTGCT |
| Suggested primer 2: | TGAATCTGCGTTACCTGCGT |