| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070551 |
| Chromosome: | chromosome 9 |
| Location: | 5232212 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407050 | MMP24 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGGTATCACTCCACAAAAAAATGTTCCCCGTCATGTACAGAATTTG |
| Internal bar code: | GATTACTGGATTTCCTTCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGGAGGAGGAGGAGGTT |
| Suggested primer 2: | CTTCCCCAACCACCATCTCC |