Insertion junction: CLIP2.070561_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.070561
Chromosome:chromosome 7
Location:4966242
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g347000 (1 of 6) IPR001245//IPR011009 - Serine-threonine/tyrosine-protein kinase catalytic domain // Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACTCCCGCTTCGTGTTCACCAATAAGGTTCTTCGTGTTCACCAGG
Internal bar code:ACTGATTTATGGTGGACTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3608
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCTGTCACGGACGGTTT
Suggested primer 2:CAGCATGAAACACACGCACA