| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070569 |
| Chromosome: | chromosome 16 |
| Location: | 3752523 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683793 | (1 of 1) K14843 - pescadillo (PES1, NOP7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTTAACTTTCAGTGCGACTAGATCCTTTCCAGGCTGGACCGTGGTCC |
| Internal bar code: | CCGTTAGTGAAGGACCTTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 31.25 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCGGTTTTATTGGTGTT |
| Suggested primer 2: | CGTTTTGCCACTCTGTGAGC |