Insertion junction: CLIP2.070569_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.070569
Chromosome:chromosome 16
Location:3752526
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683793 (1 of 1) K14843 - pescadillo (PES1, NOP7) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAATGCGACCTGGGCTGCTAGGGAATGCACCAGTGCATGAGTGTGATAG
Internal bar code:CCGTTAGTGAAGGACCTTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3167
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTTGCCACTCTGTGAGC
Suggested primer 2:GGCGCGGTTTTATTGGTGTT