| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070577 |
| Chromosome: | chromosome 2 |
| Location: | 4953735 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110300 | DNJ25 | (1 of 62) PF00226 - DnaJ domain (DnaJ); DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGTATGGCACCCGGACCGCCACGGTGGCGCGGAGAACGCAAAACGG |
| Internal bar code: | GTCCGAAATCAGCAAAATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2691 |
| LEAP-Seq percent confirming: | 49.2063 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 126 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTCAAAAGCGCCTTCCGT |
| Suggested primer 2: | GTGAGTGTGTTCGTTTGCCC |