| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070578 |
| Chromosome: | chromosome 12 |
| Location: | 5290312 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528150 | OST3 | (1 of 2) PF04756 - OST3 / OST6 family (OST3_OST6); Oligosaccharyl transferase complex subunit 3 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCACCGTATCAAGAGTCTATGCAGTGGCAACCATTTCACGAGCACAGC |
| Internal bar code: | ATCCAAGCTGTGAACGACGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5036 |
| LEAP-Seq percent confirming: | 97.5309 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTCCTGATAGCCTGACT |
| Suggested primer 2: | AGGACGCTCTGTACCCTGAT |