Insertion junction: CLIP2.070585_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.070585
Chromosome:chromosome 2
Location:8136806
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g144000 IND1 (1 of 1) PTHR10584//PTHR10584:SF201 - SUGAR KINASE // INDIGOIDINE SYNTHASE A-LIKE PROTEIN; Indigoidine synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGCGACCTGTGAAATACCAGCCGTCGCTCGAATTCGCTTTCATGTAC
Internal bar code:GACTTGCCCAAGTACGTCCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1676
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTTTCGGAGTTGGACGGC
Suggested primer 2:GAGCACTCACCTGTACCCAC