| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070603 |
| Chromosome: | chromosome 6 |
| Location: | 2315752 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267500 | (1 of 1) K02735 - 20S proteasome subunit beta 3 (PSMB3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTACCAGAACACGTCATCACAACTCCCATACCCTAGCCTCGTTCTTCT |
| Internal bar code: | GTGATTGACGTATTTGCTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1147 |
| LEAP-Seq percent confirming: | 51.0204 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACCACCCTCTCCTCC |
| Suggested primer 2: | GCATCATGGCAAATCCGGTC |