| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070609 |
| Chromosome: | chromosome 7 |
| Location: | 681638 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317300 | ROC78,MAPKKKK1,SIK1 | (1 of 1) PTHR24361:SF268 - SERINE/THREONINE-PROTEIN KINASE 3; Mitogen Activated Protein Kinase Kinase Kinase Kinase | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCAGCCAGGGCTTACATTTAAGTTGCATGTAAGTAATATCCACAGCG |
| Internal bar code: | AAAGGTAAACTTCGTTGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 815 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATCACCTTGATGGCCACA |
| Suggested primer 2: | GCAGCCGCCTGTCTGTATAT |