| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070621 |
| Chromosome: | chromosome 12 |
| Location: | 6280049 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536550 | ODA-IC1,IC78,ODA9,DIC1 | (1 of 1) K10409 - dynein intermediate chain 1, axonemal (DNAI1); Flagellar outer dynein arm intermediate chain 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGCAAATCGATATCCTAAATTGTTACTACGTGTAATGTGATGTGAAC |
| Internal bar code: | AGTACATCTATAATTTTGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1547 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTACATGGGTTGCGATC |
| Suggested primer 2: | ATGGAAAACCGTCAGCGACT |