| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070645 |
| Chromosome: | chromosome 3 |
| Location: | 2575357 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160000 | HDA3 | (1 of 3) PTHR10625//PTHR10625:SF102 - HISTONE DEACETYLASE // HISTONE DEACETYLASE 10; RPD3/HDA1 type histone deacetylase | 3'UTR |
| Cre03.g160050 | FAP184 | (1 of 1) PTHR15654:SF1 - COILED-COIL DOMAIN-CONTAINING PROTEIN 96; Flagellar Associated Protein 184 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGATCCAATGATGAGCAAGTCACGGGCGGCCCGCATGCATGTAACGCA |
| Internal bar code: | GTGGGTGCTAGAAGGCCACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3624 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 192 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGGCTCAATCGAGGAGG |
| Suggested primer 2: | CGACTCTTACGGGGATGACG |