| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070646 |
| Chromosome: | chromosome 6 |
| Location: | 7138856 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298150 | (1 of 230) IPR017986 - WD40-repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCAATAAAGATCTGCGGACGGTCTGGTTTGTGCTCGCCTGCCGCAAA |
| Internal bar code: | TCTCAGTGGTGCGCGCGTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3482 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGGCCGAGCATACATAA |
| Suggested primer 2: | CAGATGGGATCGGGGAATGG |