| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070653 |
| Chromosome: | chromosome 17 |
| Location: | 1233726 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705000 | FAP223,CDPK1 | (1 of 1) PF00069//PF00168//PF13499//PF13833 - Protein kinase domain (Pkinase) // C2 domain (C2) // EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8); Flagellar Associated Protein 223 | 5'UTR |
| Cre17.g705050 | LIC1 | (1 of 3) PF02931//PF02932 - Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD) // Neurotransmitter-gated ion-channel transmembrane region (Neur_chan_memb); Ligand-gated ion-channel | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGAGACTGTGCATGTGAGGCCTGGACTGAGGACACATCGTCGCTGGT |
| Internal bar code: | GGGGGCCCTCGCCCTCTATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4080 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCAGGGGTATGTCCAGCG |
| Suggested primer 2: | GTGACCGAGAGCACCTTGAA |