| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.070655 |
| Chromosome: | chromosome 16 |
| Location: | 4583581 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671150 | (1 of 1) PTHR12770//PTHR12770:SF22 - FAMILY NOT NAMED // UPF0420 PROTEIN C16ORF58 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCCCTGCCTGCACTGCCGCCTCCACCCGCCCTGTACGCCTCAGCTC |
| Internal bar code: | CATCTTTTCTCACTTGCGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGCCGTGTGGTGTAGA |
| Suggested primer 2: | CGCTCTTGTGCAATGGGTTT |