| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070663 |
| Chromosome: | chromosome 12 |
| Location: | 6342147 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537000 | (1 of 1) PF02041 - Auxin binding protein (Auxin_BP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTTGGGCTGCCCAGCACTTGACGTCGTACGTGCTGCAGTACATTAC |
| Internal bar code: | ATTCATAGTAGCCGATGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAATGACACAGCGGTCGT |
| Suggested primer 2: | CCAGTGGTTCGGAACAACCT |