| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070683 |
| Chromosome: | chromosome 14 |
| Location: | 1480074 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617450 | HSP22E | Heat shock protein 22E; (1 of 4) PTHR11527//PTHR11527:SF128 - SMALL HEAT-SHOCK PROTEIN HSP20 FAMILY // SUBFAMILY NOT NAMED | 3'UTR |
| Cre14.g617500 | FAP132,RSP14 | Radial Spoke Protein 14; (1 of 1) PTHR15599:SF1 - RHABDOID TUMOR DELETION REGION PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGTACGTGCTTTATATACTGCAACAACATCGCGCTCGCTGCCTCCCA |
| Internal bar code: | AAATTCAAGTATACTCGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1376 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCCTTCTTTCCCCCACC |
| Suggested primer 2: | GCATATCTTCGAGACGGCCA |