| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070684 |
| Chromosome: | chromosome 12 |
| Location: | 42554 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483550 | VPE1 | (1 of 1) K01369 - legumain (LGMN); Vacuolar processing enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTTCGTACCCCCCCCCCCCTGGACGACCGACCCTGAAGTTTAAGGCC |
| Internal bar code: | AGATCATGCAAGAAATATAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1833 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACAGCCACGGCATCAGTT |
| Suggested primer 2: | TCAAAGTTCAGGGTCGGTCG |