| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070688 |
| Chromosome: | chromosome 16 |
| Location: | 7365247 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679669 | CMS1 | 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, chloroplast precursor; (1 of 1) 2.7.7.60 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase / MEP cytidylyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGTGGCTGGAAGCATCAATGTCTTGAACTGAGGAGCATGGGCTGGGAT |
| Internal bar code: | GCCGATAGTTACTAAATTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3043 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAGGTGTACGTGGAACA |
| Suggested primer 2: | CAAACGCAACAACCTGGAGG |