| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070692 |
| Chromosome: | chromosome 4 |
| Location: | 3292111 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226900 | (1 of 1) PTHR15441:SF1 - RIBONUCLEASE P PROTEIN SUBUNIT P14 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCCAAACGCAACTGGTCGTAGGCCGTACACCGCATGAGCACATGATT |
| Internal bar code: | GGGGGTGTCTAAGCGATACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2583 |
| LEAP-Seq percent confirming: | 55.1724 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGCCTCAATACTCGTTA |
| Suggested primer 2: | GTCGTGATGCCGTGTCATTG |