Insertion junction: CLIP2.070694_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.070694
Chromosome:chromosome 12
Location:3644741
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g513400 EBG1 Endo-1%252C3(4)-beta-glucanase; (1 of 1) 3.2.1.6 - Endo-1,3(4)-beta-glucanase / Laminarinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAAGCAGCGCCGCACCCATGAGATAGTAGCCGTAGTGGCTGCAGGGAT
Internal bar code:AATACACGGAAGCGTCACTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:2257
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCTTATTACGGCGCCTCG
Suggested primer 2:CGGCTCGTAAACATGCATGG