| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070698 |
| Chromosome: | chromosome 8 |
| Location: | 4454782 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385700 | SNAPG1 | (1 of 1) PTHR13768:SF2 - GAMMA-SOLUBLE NSF ATTACHMENT PROTEIN; Gamma-SNAP, SNARE complex protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCGCGACAACGCGTAACCCACAAAATGCACCCACCTGGAACGAAGCC |
| Internal bar code: | AGGCTCAGGGATTAACTGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3329 |
| LEAP-Seq percent confirming: | 81.4286 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCGCTACTGACTGGTTT |
| Suggested primer 2: | TGCAAGGCAGTCTACACAGG |