| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070702 |
| Chromosome: | chromosome 16 |
| Location: | 1635702 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654350 | SPL4 | (1 of 1) K13102 - DNA/RNA-binding protein KIN17 (KIN); Splicing factor-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCCTTAAGATGAAATGGGCAGGTTACTGAAGGCTCCACGCGGCACCT |
| Internal bar code: | AGCACACAGTTGTCTCTCCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCCACATGAAATTGGGA |
| Suggested primer 2: | GTTCGTCAAGTACCTGGGCA |