Insertion junction: CLIP2.070713_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.070713
Chromosome:chromosome 3
Location:5678561
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g186600 5'UTR
Cre03.g186650 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTATGCTAGAAAGCAGATGCAGGCGAAGAGGTTGGCGACTGATGTTCCA
Internal bar code:GGGTGTTTGGTCCCAAATGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:6374
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:143
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:143

Suggested primers for confirmation by PCR

Suggested primer 1:GTTAGGGGCGTGTGTGATGA
Suggested primer 2:ACTGACGCTAGCAGCTGTTT