| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070716 |
| Chromosome: | chromosome 3 |
| Location: | 5086919 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180300 | SIR3 | (1 of 1) 1.8.1.2 - Assimilatory sulfite reductase (NADPH) / Sulfite reductase (NADPH); Sulfite reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACATTGCGGTAATAGCCACACAGCTAAATCAACTCACAACGCACAC |
| Internal bar code: | TTTTCGCGTCAATCCTTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5361 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGTACGTGTGACCAGCA |
| Suggested primer 2: | ACAGACGCAACGTGCTAGAA |