Insertion junction: CLIP2.070718_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070718
Chromosome:chromosome 7
Location:4576417
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGCACACACACACACACGCGCGCATCACGTCGTCGCTACTGCTTCC
Internal bar code:ACTATGCGAGTAGTTTTTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:303
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGACTGCAAGCAAGGACT
Suggested primer 2:TACCCCCGCCCTTCATCTTA