| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070721 |
| Chromosome: | chromosome 17 |
| Location: | 876630 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702400 | PUS5 | (1 of 1) 3.5.4.26//5.4.99.28 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase // tRNA pseudouridine(32) synthase / Pseudouridine synthase RluA; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAGGACCTTCCGCACACCCTCGCAAACTTCGGCAACATGAGGGCCCGC |
| Internal bar code: | CGTGTTTGTTGTTACTTTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATACACACACACCCCGA |
| Suggested primer 2: | GTGGCTGGGAAGATGGGTAC |