| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070722 |
| Chromosome: | chromosome 13 |
| Location: | 4229266 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACAAGCACTCGTTTCGAATATCTTTACAAGTGTCTGTTCAATATCGT |
| Internal bar code: | AGTAGGTTCGGGAACAGTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 6.89655 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATGAACGTGTCCTGAA |
| Suggested primer 2: | GCGTCAGTCGAGTCTCTAGC |