| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070742 |
| Chromosome: | chromosome 7 |
| Location: | 4549170 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344000 | (1 of 1) PTHR31988:SF1 - 9-O-ACETYL-N-ACETYLNEURAMINIC ACID DEACETYLASE-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAACGCCGCCGCCTACGGCGCCCGCTTCGCCGCCTTCCTGGCGGCGG |
| Internal bar code: | CGGGAGGAATTTTATTGTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTTACTTGCCCGTTGC |
| Suggested primer 2: | CGGAAAGTCTGGCAGTGACT |