| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070742 |
| Chromosome: | chromosome 14 |
| Location: | 913405 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613350 | (1 of 1) K14016 - ubiquitin fusion degradation protein 1 (UFD1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCTACGTGGTGGCAAAGGGGTTGCAAAGGGGTGGTGTGGCGATGGGG |
| Internal bar code: | GAGGCTCATTTGGGGAGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1397 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGATTGGAAGGAGACC |
| Suggested primer 2: | TGAGTGAAGCTTCGGGTGAC |