Insertion junction: CLIP2.070747_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.070747
Chromosome:chromosome 17
Location:4513201
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g802092 (1 of 1) PF00050//PF05548//PF07648 - Kazal-type serine protease inhibitor domain (Kazal_1) // Gametolysin peptidase M11 (Peptidase_M11) // Kazal-type serine protease inhibitor domain (Kazal_2) gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCCAGTGTGACCGGTGACAGTGTGACAGTCCTTCACGAAAACCATCG
Internal bar code:CGGAATGCACTTACGTGAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:849
LEAP-Seq percent confirming:58.3333
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAAAGCGTACGGTTGGCT
Suggested primer 2:GTGCACAATACAGTAGCGCG