| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070750 |
| Chromosome: | chromosome 3 |
| Location: | 1940561 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155200 | CHM1 | Chorismate mutase; (1 of 1) 5.4.99.5 - Chorismate mutase / Hydroxyphenylpyruvate synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGACTGCCCGCCTGCCAGCTAAGCCTTCTGACAGGGAGGTAAGCAAT |
| Internal bar code: | AGGCATACTCATCTGTAGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1932 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCTATAAGGGCCAGTC |
| Suggested primer 2: | TAATCCCAGAGCTAAGCGCG |