| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070763 |
| Chromosome: | chromosome 1 |
| Location: | 4820851 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033550 | PDI2 | (1 of 1) PTHR18929:SF87 - PROTEIN C03H12.1, ISOFORM A; Protein disulfide isomerase 2 | outside_mRNA |
| Cre01.g800091 | (1 of 1) K18663 - activating signal cointegrator complex subunit 3 [EC:3.6.4.12] (ASCC3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGAGCCCATCCTGGCGGATTTCGGGAAGCGCCCCACAAGCAGAAATA |
| Internal bar code: | GTTAGAGCCTTCAAAGCTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1194 |
| LEAP-Seq percent confirming: | 31.5789 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAAAGTGCCAGACCCCA |
| Suggested primer 2: | ACGGTTTTGAGCTAGCGACA |