Insertion junction: CLIP2.070770_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070770
Chromosome:chromosome 1
Location:2336267
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGCGTCTCAGACCTGAGACGCTGAGCAAGTCAGCCTTAGCTATTGGA
Internal bar code:CGATGACAGTCTCTTTCGTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3255
LEAP-Seq percent confirming:86.3354
LEAP-Seq n confirming:139
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:161

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTCAGTAGCACGCAAGA
Suggested primer 2:CAGGTGTGAAGTACGGCAGT