| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070779 |
| Chromosome: | chromosome 3 |
| Location: | 8304311 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199250 | CYG51 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
| Cre03.g199300 | AHA1 | (1 of 1) PF08327//PF09229 - Activator of Hsp90 ATPase homolog 1-like protein (AHSA1) // Activator of Hsp90 ATPase, N-terminal (Aha1_N); Activator of HSP90 ATPase homolog 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGCTGGCCTCGTAAACCAGTCCTGTGATCCCGTGTGGTTCTGGCCA |
| Internal bar code: | TGACTCGGCGTCTCCGTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3830 |
| LEAP-Seq percent confirming: | 81.2081 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 149 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTTTCCTGCTGAGACCA |
| Suggested primer 2: | GTAGGGACACACTGTGGTGG |